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shearase  (Zymo Research)


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    Structured Review

    Zymo Research shearase
    Shearase, supplied by Zymo Research, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shearase/dsDNA+Shearase+(Includes+Matching+DCC-5+Kit)/10__1091_slash_mbc__e25___05___0246-331-20-22
    Average 93 stars, based on 37 article reviews
    shearase - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in <i>Schizosaccharomyces pombe</i>
    Article Snippet: 1 Title Page Title: Induction of post-meiotic DNA double-strand breaks by the Pnu1 endonuclease in

    Article Title: Methods and compositions for treating and diagnosing autoimmune diseases
    Article Snippet: Other enzymatic methods include the use of Vvn nuclease alone or Serratia nuclease, or DNase I, or other nuclease in the art such as ShearaseTM (Zymo Research, Irvine, Calif.) or Ion ShearTM (Life Technologies, Grand Island, N.Y.).

    Purification:

    Article Title: Genomics of a killifish from the Seychelles islands supports transoceanic island colonization and reveals relaxed selection of developmental genes
    Article Snippet: Forty μL SPRI beads (1mL SeraMag GE Healthcare, 65152105050250 beads in 100mL of PEG8000 20%, NaCl 2.5M, Tris-HCl pH=8.0 10mM, EDTA 1mM, Tween20 0.05%) were added directly to the mix to purify DNA with two washes by 80% ethanol. .. For library preparation, 250ng of genomic DNA was digested with 0.665μL of Shearase (Zymo) in a 10 μL volume at 42°C for 20min, inactivated at 65°C and purified with 0.8 volume of SPRI beads. .. End-repairing and A-tailing were performed by incubating in 25μL of repair mix (NEB End-repair buffer 2.5 μL, Klenow fragment with 3’-5’ exonuclease activity 0.5 μL, Taq polymerase 0.5 μL, T4 polynucleotide kinase 0.2 μL) at 25°C for 30min and 75°C for 30min, then purified with 1 volume of SPRI beads.



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